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KMID : 0364820220580040266
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Korean Journal of Microbiology
2022 Volume.58 No. 4 p.266 ~ p.274
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Jejuia spongiicola sp. nov., isolated from Callyspongia elongata§
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Kim Jeong-Hyeon
Kim Jae-Yeon
Park Jin-Sook
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Abstract
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A Gram-stain-negative, rod-shaped, non-flagella, non-gliding, obligate aerobic, and pale orange bacterium, strain 2012CJ34-3T, was isolated from a sponge sample of Callyspongia elongata from Chuja-myeon, Jeju-si, Jeju-do, Republic of Korea. On the basis of 16S rRNA gene sequencing, strain 2012CJ34-3T appeared closely related to Gaetbulibacter marinus KCTC 23081T (96.19%), Gaetbulibacter lutimaris KCTC 23716T (95.76%), Jejuia marina KCTC 42342T (95.70%), and Gaetbulibacter aquiaggeris KCTC 42198T (94.99%). Although the strain showed higher 16S rRNA gene similarity to Gaetbulibacter spp., phylogenetic analyses showed that it belongs to genus Jejuia. The average nucleotide identity and digital DNA-DNA hybridization between strain 2012CJ34-3T and Jejuia pallidilutea DSM 21165T were 75.0% and 19.20%, respectively. Growth occurs at 10?30°C on MA medium in the presence of 1?10% NaCl (w/v) and at pH 6.0?8.0. The DNA G + C content of the genomic DNA was 31.60 mol%, and menaquinone-6 (MK-6) was the major respiratory quinone. The major cellular fatty acids (> 5%) were C15:0 iso, C15:1 iso G, C15:0 iso 3OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The polar lipids consisted of phosphatidylethanolamine, unidentified aminophospholipid, unidentified aminolipid, unidentified glycolipid, and two unidentified lipids. Physiological and biochemical characteristics indicated that strain 2012CJ34-3T represents a novel species of the genus Jejuia, for which the name Jejuia spongiicola sp. nov. is proposed. The type strain is 2012CJ34-3T (= KACC 22642T = LMG 32583T).
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KEYWORD
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Callyspongia elongat, Flavobacteriaceae, Jejuia spongiicola, polyphasic taxonomy, sponge, 16S rRNA gene sequence
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